year 19, Issue 74 (6-2020)                   J. Med. Plants 2020, 19(74): 163-176 | Back to browse issues page


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Fathi N, Tafvizi F, Mirzaie A. Antibacterial and anti-cancer activities of Artemisia turcomanica extract on gastric cancer cell line (AGS) and its interaction on cyclin D1 and cyckin E genes. J. Med. Plants 2020; 19 (74) :163-176
URL: http://jmp.ir/article-1-2392-en.html
1- Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran
2- Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran , farzanehtafvizi54@gamil.com
3- Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
Abstract:   (3061 Views)
Background: The use of medicinal herbs in traditional medicine has been important and many of these plants have antioxidant, anti-inflammatory, antimicrobial and anti-cancer effects. Objective: The aim of this study was to evaluate the antibacterial and anti-cancer effects of Artemisia turcomanica extract on gastric cancer cell line (AGS) and its effect on expression of cyclin D1 and cyclin E genes. Methods: At first the alcoholic extract of A. turcomanica was prepared and its antibacterial effect was evaluated using MIC (Minimum Inhibitory Concentration) method. Subsequently, the cytotoxic effect of extract on AGS cell line was studied using MTT method after 24, 48 and 72 hours. Moreover, the apoptotic effect of extract was determined via annexin FITC-V and PI staining. Finally, the gene expression of cyclin D1 and cyclin E were measured using Real Time PCR method. Results: The antibacterial results show that the A. turcomanica extract had significant effect on Escherichia coli. In addition, the results of MTT assay indicated that the maximum cytotoxic effect of A. turcomanica extract was observed in 24 h and over time, its cytotoxic effects were decreased. The maximum apoptotic effect and minimum necrosis effects was observed in 48 h. Also, the Real Time PCR results reveal that the gene expression of cyclin D1 and cyclin E1 was down-regulated. Conclusion: The results of this study show that the
A. turcomanica extract had cytotoxic and apoptotic effects on AGS cell line and it can be used as anticancer candidate in cancer therapy and prevention after further studies.
Full-Text [PDF 1136 kb]   (3279 Downloads)    
Type of Study: Research |
Received: 2018/12/15 | Accepted: 2019/03/18 | Published: 2020/07/21

References
1. Bent S. Herbal medicine in the United States: review of efficacy, safety, and regulation: grand rounds at University of California, San Francisco Medical Center. J. Gen. Intern. Med. 2008; 23 (6): 854 - 9. [DOI:10.1007/s11606-008-0632-y]
2. Bent S1 and Ko R. Commonly used herbal medicines in the United States: a review. Am. J. Med. 2004; 116 (7): 478 - 85. [DOI:10.1016/j.amjmed.2003.10.036]
3. Firenzuoli F and Gori L. Herbal medicine today: clinical and research issues. eCAM. 2007; 4 (Suppl 1): 37 - 40. [DOI:10.1093/ecam/nem096]
4. Bora KS1 and Sharma A. The genus Artemisia: a comprehensive review. Pharm. Biol. 2011; 49 (1): 101 - 9. [DOI:10.3109/13880209.2010.497815]
5. Tan RX, Zheng WF and Tang HQ. Biologically active substances from the genus Artemisia. Planta Med. 1998; 64 (4): 295 - 302. [DOI:10.1055/s-2006-957438]
6. Blackadar CB. Historical review of the causes of cancer. WJCO. 2016; 10; 7 (1): 54 - 86. [DOI:10.5306/wjco.v7.i1.54]
7. Alberto d'Onofrio, Chiara Mazzetta, Chris Robertson, Michel Smans, Peter Boyle, Mathieu Boniol. Maps and atlases of cancer mortality: a review of a useful tool to trigger new questions. E cancer medical science 2016; 10: 670. [DOI:10.3332/ecancer.2016.670]
8. Sitarz R, Skierucha M, Mielko J, Offerhaus GJA, Maciejewski R and Polkowski WP. Gastric cancer: epidemiology, prevention, classification, and treatment. Cancer Manag. Res. 2018; 7; 10: 239 - 48. [DOI:10.2147/CMAR.S149619]
9. Charalampakis N, Economopoulou P, Kotsantis I, Tolia M, Schizas D, Liakakos T, Elimova E, Ajani JA and Psyrri A. Medical management of gastric cancer: a 2017 update. Cancer Med. 2018; 7 (1): 123 - 33. [DOI:10.1002/cam4.1274]
10. Urruticoechea A, Alemany R, Balart J, Villanueva A, Viñals F and Capellá G. Recent advances in cancer therapy: an overview. Curr. Pharm. Des. 2010; 16 (1): 3 - 10. [DOI:10.2174/138161210789941847]
11. Ortiz R1, Melguizo C, Prados J, Álvarez PJ, Caba O, Rodríguez-Serrano F, Hita F and Aránega A. New gene therapy strategies for cancer treatment: a review of recent patents. Recent Pat. Anti-Cancer Drug Discov. 2012; 7 (3): 297-312. [DOI:10.2174/157489212801820093]
12. Qie S and Diehl JA. Cyclin D1, cancer progression, and opportunities in cancer treatment. J. Mol. Med (Berl). 2016; 94 (12): 1313-26. [DOI:10.1007/s00109-016-1475-3]
13. Möröy T and Geisen C. Cyclin E. IJBCB. 2004; 36 (8): 1424-39. [DOI:10.1016/j.biocel.2003.12.005]
14. Alao JP. The regulation of cyclin D1 degradation: roles in cancer development and the potential for therapeutic invention. Mol. Cancer. 2007; 2; 6: 24. [DOI:10.1186/1476-4598-6-24]
15. Taghizadeh Rabe SZ, Mahmoudi M, Ahi A and Emami SA. Antiproliferative effects of extracts from Iranian Artemisia species on cancer cell lines. Pharm. Biol. 2011; 49 (9): 962-9. [DOI:10.3109/13880209.2011.559251]
16. Satyavani K, Gurudeeban S, Ramanathan T and Balasubramanian T. Toxicity study of silver nanoparticles synthesized from Suaeda monoica on Hep-2 Cell Line. AJMB. 2012; 4 (1): 35-9.
17. Badmus JA, Ekpo OE, Hussein AA, Meyer M and Hiss DC. Antiproliferative and apoptosis induction potential of the methanolic leaf extract of Holarrhena floribunda (G. Don). eCAM. 2015; 2015: 756482. [DOI:10.1155/2015/756482]
18. Pozarowski P, Darzynkiewicz Z. Analysis of cell cycle by flow cytometry. Methods Mol. Biol. 2004; 281: 301-11. [DOI:10.1385/1-59259-811-0:301]
19. Hirata Y, Maeda S, Mitsuno Y, Akanuma M, Yamaji Y, Ogura K, Yoshida H, Shiratori Y and Omata M. Helicobacter pylori activates the cyclin D1 gene through mitogen-activated protein kinase pathway in gastric cancer cells. Infect Immun. 2001; 69 (6): 3965-71. [DOI:10.1128/IAI.69.6.3965-3971.2001]
20. Wypij M, Czarnecka J, Świecimska M, Dahm H, Rai M and Golinska P. Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain. World J. Microbiol. Biotechnol. 2018; 5; 34 (2): 23. [DOI:10.1007/s11274-017-2406-3]
21. Mahdi-Pour B1, Jothy SL, Latha LY, Chen Y and Sasidharan S. Antioxidant activity of methanol extracts of different parts of Lantana camara. Asian Pac. J. Trop. Biomed. 2012; 2 (12): 960-5. [DOI:10.1016/S2221-1691(13)60007-6]
22. Carcas LP. Gastric cancer review. J. Carcinog. 2014; 19; 13: 14. [DOI:10.4103/1477-3163.146506]
23. Tsuruo T1, Naito M, Tomida A, Fujita N, Mashima T, Sakamoto H and Haga N. Molecular targeting therapy of cancer: drug resistance, apoptosis and survival signal. Cancer Sci. 2003; 94 (1): 15-21. [DOI:10.1111/j.1349-7006.2003.tb01345.x]
24. Gordanian B, Behbahani M, Carapetian J and Fazilati M. In vitro evaluation of cytotoxic activity of flower, leaf, stem and root extracts of five Artemisia species. Res. Pharm. Sci. 2014; 9 (2): 91-6.
25. Mousavi B, Tafvizi F, Zaker Bostanabad S. Green synthesis of silver nanoparticles using Artemisia turcomanica leaf extract and the study of anti-cancer effect and apoptosis induction on gastric cancer cell line (AGS). Artif Cells Nanomed Biotechnol. 2018; 23: 1-12. [DOI:10.1080/21691401.2018.1430697]
26. Salehi S, Shandiz SA, Ghanbar F, Darvish MR, Ardestani MS, Mirzaie A and Jafari M. Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties. Int. J. Nanomedicine 2016; 11: 1835-46. [DOI:10.2147/IJN.S99882]
27. Johnson AS, Obot I.B and Ukpong US. Green synthesis of silver nanoparticles using Artemisia annua and Sida acuta leaves extract and their antimicrobial, antioxidant and corrosion inhibition potentials. J. Mater. Environ. Sci. 2014; 5: 3: 899-906.
28. Karimi N, Rashedi J, Mahdavi Poor B, Arabi S, Ghorbani M, Tahmasebpour N and Asgharzadeh M. Cytotoxic effect of rosemary extract on gastric adenocarcinoma (AGS) and esophageal squamous cell carcinoma (KYSE30) cell lines. Gastroenterol. Hepatol. Bed Bench. 2017; 10 (2): 102-7.
29. Zhang L, Wu H, Sun G, Xu X, Sun X and Cao L. Trichloromethane fraction of Incarvillea compacta induces lytic cytotoxicity and apoptosis in Epstein-Barr virus-positive gastric cancer AGS cells. BMC Complement. Altern. Med. 2016; 16: 344. [DOI:10.1186/s12906-016-1331-6]

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