سال 19، شماره 74 - ( 3-1399 )                   سال 19 شماره 74 صفحات 144-129 | برگشت به فهرست نسخه ها


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1- گروه علوم باغبانی، دانشگاه تبریز، تبریز،ایران
2- گروه علوم باغبانی، دانشگاه تبریز، تبریز،ایران ، n.mahna@gmail.com
3- گروه بیوتکنولوژی و اصلاح نباتات، دانشگاه کشاورزی و منابع طبیعی ساری، مازندران، ایران
4- گروه بیوتکنولوژی و اصلاح نباتات، پژوهشکده ژنتیک دانشگاه کشاورزی و منابع طبیعی دانشگاه ساری، مازندران،ایران
چکیده:   (3327 مشاهده)
مقدمه: امروزه روش‌های بیوتکنولوژی مانند کشت ریشه مویین و استفاده از محرک‌ها، پتانسیل قابل توجهی برای تولید متابولیت‌های ثانویه دارند. زوفا، گیاهی با مواد مؤثره دارویی وآنتی‌اکسیدان‌هایی مانند ترکیبات فنلی  است که ویژگی‌های ضد‌جهشی، ضد‌سرطانی و همچنین کاهش قند خون را دارد. هدف: مطالعه اثر محرک نانوذرات نقره بر رشد و میزان ترکیبات فنلی و آنتی‌اکسیدانی ریشه‌های مویین تراریخت در دو گونه زوفای باریک برگ و دارویی، در شرایط کشت بافت می‌باشد. روش بررسی: در این مطالعه از چهار غلظت 0، 1/0، 2/0، 3/0 میلی‌گرم بر لیتر نانوذرات نقره و همچنین از سویه‌ی ATCC15834 باکتری‌Agrobacterium rhizogenes  برای االقای ریشه مویین تراریخت استفاده شد. عصاره‌گیری از نمونه‌ها به روش اولتراسونیک انجام گرفت. سپس سـنجش میـزان فنل کل به روش اسپکتروفتومتری و در نهایت فعالیت آنتی‌اکسیدانی عصاره با استفاده از روش مهار رادیکال آزاد 2 و '2 - دی فنیل 1- پیکریل هیدرازیل (DPPH) اندازه‌گیری شد. نتایج: اثر تحریکی نانوذرات نقره بر صفات مورد آزمایش در دو گونه زوفا در سطح یک درصد معنی‌دار است. بیشترین میزان وزن خشک و وزن تر ریشه مویین به ترتیب با مقدار 8/3 و 16 گرم بر لیتر در گونه باریک برگ (H. angustifolius) و 2/3 و 14 گرم بر لیتر در گونه دارویی (Hyssopus officinalis) مشاهده شد، که همگی در غلظت 1/0 میلی‌گرم بر لیتر نانوذرات نقره حاصل شدند. بیشترین میزان آنتی‌اکسیدان در زوفای باریک برگ و زوفای دارویی به ترتیب 81/90 و 84/89 درصد به دست آمد. نتیجه‌گیری: به طور کلی نانوذرات نقره در این آزمایش اثر مطلوبی بر رشد ریشه مویین گیاه زوفا داشت و همجنین، در غلظت 1/0 میلی‌گرم، میزان تولید مواد فنلی و آنتی‌اکسیدان را افزایش داد.
متن کامل [PDF 653 kb]   (1782 دریافت)    
نوع مطالعه: پژوهشی | موضوع مقاله: بيوتكنولوژی
دریافت: 1397/8/1 | پذیرش: 1397/12/7 | انتشار: 1399/4/31

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