year 19, Issue 74 (6-2020)
J. Med. Plants 2020, 19(74): 177-191 |
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Sharifiyan F, Mirjalili S A, Fazilati M, Poorazizi E, Habibollahi S. Cytotoxic effect of hydro-alcoholic extract of pomegranate (Punica granatum L.) flowers and isolated ursolic acid on B16f10 melanoma cells. J. Med. Plants 2020; 19 (74) :177-191
URL: http://jmp.ir/article-1-2248-en.html
URL: http://jmp.ir/article-1-2248-en.html
Fatemeh Sharifiyan1 
, Seyed Abbas Mirjalili * 2, Mohammad Fazilati3 , Elahe Poorazizi4 , Saeed Habibollahi5
, Seyed Abbas Mirjalili * 2, Mohammad Fazilati3 , Elahe Poorazizi4 , Saeed Habibollahi5
1- Department of Biology, Payame Noor University (PNU), Iran
2- Imam Khomeini Higher Education Center, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran , abmirjalili@gmail.com
3- Imam Khomeini Higher Education Center, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
4- Department of Biochemistry, Najafabad Branch, Islamic Azad University, Najafabad, Iran
5- Department of Chemistry, Payame Noor University (PNU), Iran
2- Imam Khomeini Higher Education Center, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran , abmirjalili@gmail.com
3- Imam Khomeini Higher Education Center, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
4- Department of Biochemistry, Najafabad Branch, Islamic Azad University, Najafabad, Iran
5- Department of Chemistry, Payame Noor University (PNU), Iran
Abstract: (3151 Views)
Background: Melanoma is the most serious form of skin cancer and it has the highest rate of growth among different types of cancers. Ursolic acid (UA), a natural pentacyclic triterpenoid, is a major bioactive compound in several traditional medicinal plants including pomegranate (Punica granatum L.) flower. UA has very important biological and pharmacological functions. Objective: In this study, cytotoxic effects of ethanol extract of pomegranate flowers and isolated UA against B16f10 mouse melanoma cells were investigated. Methods: The isolation of UA from pomegranate flowers was done by using ultrasound-assisted extraction followed by normal-phase flash chromatography. The chemical structure of isolated UA was identified by GC-MS, IR and 1H-NMR spectra. The ethanol extract of pomegranate flowers and isolated UA were then tested for the cytotoxicity against B16f10 mouse melanoma cell lines. Cell viability was determined using MTT assay. Results: Ursolic acid was successfully isolated from pomegranate flowers by using normal-phase flash chromatography. After one flash chromatography run, the purity of UA reached more than 97% with a total yield of 0.1 % of dried pomegranate flower powder. Treatment of B16f10 cells with ethanol extract of pomegranate flowers and isolated UA exhibited significant anti-proliferative activity after incubation for 48 and 72 hours. It was observed for pomegranate flower extract in a concentration and time dependent manner. Conclusion: The results suggested that pomegranate flower extract and isolated UA can be encouraging compounds in the prevention and treatment of melanoma.
Type of Study: Research |
Subject:
Medicinal Plants
Received: 2018/08/29 | Accepted: 2019/04/6 | Published: 2020/07/21
Received: 2018/08/29 | Accepted: 2019/04/6 | Published: 2020/07/21
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