سال 17، شماره 65 - ( English 1396 )
سال 17 شماره 65 صفحات 82-73 |
برگشت به فهرست نسخه ها
1- Department of Horticultural Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2- دانشگاه فردوسی مشهد
3- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
4- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2- دانشگاه فردوسی مشهد
3- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
4- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
چکیده: (30598 مشاهده)
Background: Papaver bracteatum Lindl. Commonly known as Iranian poppy is an important medicinal plant due to the presence of benzylisoquinoline alkaloids.
Objective: To evaluate the hairy root culture as a novel method for thebaine production.
Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes (ATCC15834, C318, A13, 9453 and A4) were used by means of Sonication-Assisted Agrobacterium-mediated Transformation (SAAT).
Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
Conclusion: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
Objective: To evaluate the hairy root culture as a novel method for thebaine production.
Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes (ATCC15834, C318, A13, 9453 and A4) were used by means of Sonication-Assisted Agrobacterium-mediated Transformation (SAAT).
Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
Conclusion: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
واژههای کلیدی: Papaver bracteatum، Lindl. Hairy root، Sonication-assisted، Agrobacterium-mediated transformation، Thebaine
نوع مطالعه: پژوهشی |
موضوع مقاله:
بيوتكنولوژی
دریافت: 1396/6/19 | پذیرش: 1396/10/23 | انتشار: 1396/12/19
دریافت: 1396/6/19 | پذیرش: 1396/10/23 | انتشار: 1396/12/19
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