[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 4, Issue 64 And S11 (1-2018) ::
JMP 2018, 4(64 And S11): 107-122 Back to browse issues page
Characterization of Effective Parameters on the Synthesized Gold Nanoparticles and Investigating their Antimicrobial Activities Using Aqueous Extract of Hibiscus sabdariffa L.
M Mehrzadeh 1, J Valizadeh 2, A Ghasemi 3
1- (M.Sc.) 1- Department of Chemistry, Faculty of Science, University of Sistanand & Baluchestan, Zahedan, Iran
2- (Ph.D.) 2- Department of Biology, Faculty of Science, University of Sistanand Baluchestan, Zahedan, Iran
3- (B.Sc.) 2- Department of Biology, Faculty of Science, University of Sistanand Baluchestan, Zahedan, Iran
Abstract:   (891 Views)
Background: During the last decade research have been focussed on developing simple, clean, non-toxic, cost effective and ecofriendly protocols for synthesis of nanoparticles.
Objective: The current study aim to characterization and biosynthesis of gold nano particles (Au NPs) using calyx aqueous extract of Hibiscus sabdariffa L. and study of their antimicrobial activity.
Methods: Gold nano particles (AuNPs) were biosynthesized by using HAuCl4.3H2O and the calyx aqueous extract of Hibiscus sabdariffa L. In order to gain the best AuNPs with a uniform shape and size, parameters affecting on synthesis such as: volume of plant extract, pH of the reaction, concentration of HAuCl4.3H2O solution, time of reaction were investigated and optimized by UV-Vis spectrophotometry. Moreover, transmission electron microscopy (TEM) and X-ray diffraction (XRD) were used for further characterization of the nanoparticles. Finally, the antibacterial properties of gold nanoparticles were evaluated by disc diffusion method.
Results: The results of transmission electron image identified the formation of spherical nanoparticles with a size range of 5-10nm. A sharp peak with a maximum absorbance of 570nm was observed which is the characteristic wavelength of gold nanoparticles. The highest inhibition were obtained with the gold nanoparticles against Hafnia alvei bacteria.
Conclusion: In this study, the gold nanoparticles synthesized by calyx aqueous extract of
H. sabdariffa L. and they have relatively good antibacterial activity against Gram-negative bacteria. 
 
Keywords: : Hibiscus sabdariffa L., Antibacterial activity, Biosynthesis, Gold Nanoparticles
Full-Text [PDF 640 kb]   (385 Downloads)    
Type of Study: Research | Subject: Vioshima
Received: 2017/12/31 | Accepted: 2017/12/31 | Published: 2017/12/31
Add your comments about this article
Your username or Email:

Write the security code in the box >


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mehrzadeh M, Valizadeh J, Ghasemi A. Characterization of Effective Parameters on the Synthesized Gold Nanoparticles and Investigating their Antimicrobial Activities Using Aqueous Extract of Hibiscus sabdariffa L.. JMP. 2018; 4 (64) :107-122
URL: http://jmp.ir/article-1-1980-en.html


Volume 4, Issue 64 And S11 (1-2018) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3638