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:: Volume 4, Issue 64 And S11 (1-2018) ::
JMP 2018, 4(64 And S11): 83-90 Back to browse issues page
Cytotoxic Effects of Allium affine Ledeb Butanolic Fraction on Breast and Ovary Cancer Cell Lines
M Kazemi 1, B Zolfaghari 2, M Keivanloo Shahrestanaki 3, M Sadeghi Dinani * 2
1- School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
2- Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
3- Department of clinical Biochemistry, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract:   (901 Views)
Background: Among the secondary metabolites isolated from Allium species, steroidal saponins are more important for their cytotoxic activities on a variety of cancer cell lines. Current study has investigated the cytotoxic activity of saponin-riched butanolic fraction of Allium affine, an important edible Allium species of Iran.
Objective: This study was conducted to evaluate cytotoxic activity of saponin-riched fraction of Allium affine Ledeb on breast and ovarian cancer cell lines.  
Methods: Bulbs of the plant were extracted respectively by hexane, chloroform, chloroform-methanol (9-1) and methanol in a stepwise method with increasing solvent polarity. The methanol extract was then partitioned between water and butanol and the final dried butanolic fraction was used for further cytotoxicity assay. MCF-7, MDA-MB231 and OVCAR-3 cell lines were tested for cytotoxic activity, using different concentrations of butanolic fraction (0.1-200 µg/ml) by MTT assay. After the incubation time (48 h), the percentage of the viable cells was determined by ELISA reader instrument in 570 nm.
Results: All of the cell lines tested in this investigation exhibited a dose-dependent decrease in succinate dehydrogenase activity against the control and significant dose-dependent inhibition of the growth in all concentrations and cell lines were observed (P<0.01). The most cytotoxic activity was observed for OVCAR-3 cell line (IC50= 7.13±0.94 µg/ml).
Conclusion: The butanolic fraction of A. affine exhibited a significant cytotoxic activity on investigated cell lines, especially OVCAR-3, which is in agreement with other studies conducted on different Allium species. According to the results, it seems that the butanolic fraction of this plant contains cytotoxic components, especially steroidal saponins, and the phytochemical study of the constituents is suggested.
Keywords: Allium affine, Breast Cancer, Cytotoxicity, Ovarian Cancer, Steroidal Saponin
Full-Text [PDF 352 kb]   (259 Downloads)    
Type of Study: Research | Subject: Pharmacognosy
Received: 2017/12/31 | Accepted: 2017/12/31 | Published: 2017/12/31
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Kazemi M, Zolfaghari B, Keivanloo Shahrestanaki M, Sadeghi Dinani M. Cytotoxic Effects of Allium affine Ledeb Butanolic Fraction on Breast and Ovary Cancer Cell Lines. JMP. 2018; 4 (64) :83-90
URL: http://jmp.ir/article-1-1978-en.html


Volume 4, Issue 64 And S11 (1-2018) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
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