Background: Artemisia persica is a common species in South East Iran and A. persica is the only species in this genus and the species name persica means from Iran. Objective: Artemisia persica have been used in traditional medicine to treat various infections and inflammations. It is an effective remedy for S. aureus and B. subtilis bacterial infection. Methods: The selected methanol extracts were screened against two strain of bacteria Staphylococcus aureus and Bacillus subtilis at seven different extracts concentrations (5, 25, 100, 150, 200, 300 and 400 µg/ml) using disc diffusion and wheel assay methods. The minimum inhibitory and bactericidal concentrations (MIC, MBC and Death kinetic) of the active extracts were tested using MH broth-dilution methods. Results: For standard strains of S. aureus the extracts for MIC were range 100 mg/ml and MBC range were 150-200 µg/ml. For B. subtilis standard strain the MIC extracts range were150-200 µg/ml and MBC were 400 µg/ml, Kinetic of death were 4 hours for B. subtilis compared to 5 hours for S. aureus. Conclusion: These results suggest that extracts of Artemisia persica may have the antibacterial activity against some pathogenic bacteria. It has an antibacterial action and inhibiting the growth of Staphylococcus aureus and Bacillus subtilis and it may be attributed to its content of the artemisinin, tannins, saponin, alfa- pinene and camphor. From this study it can be concluded that many extracts possess antibacterial activity Plant extracts have been used for many pharmaceuticals, alternative medicine and natural therapies.