year 16, Issue 63 (8-2017)
J. Med. Plants 2017, 16(63): 92-107 |
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1- Medicinal Plants Research center, Institute of Medicinal Plants, ACECR, Karaj, Iran , Naghdibadi@yahoo.com
2- Department of Plant Breeding and Biotechnology, University of agricultural science and natural resources Gorgan , Gorgan,Iran
3- Medicinal Plants Research center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2- Department of Plant Breeding and Biotechnology, University of agricultural science and natural resources Gorgan , Gorgan,Iran
3- Medicinal Plants Research center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Abstract: (1118 Views)
Background: Ferulago angulata is an important medicinal plant and endemic to Iran which is an endangered Plant.
Objective: The aim of this study was to achieve an efficient method for propagation of this plant and evaluation of essential oil content and composition in habitat.
Metthods: Essential oils were extracted by hydrodistillation using Clevenger-type apparatus and its composition was analyzed by the GC and GC-MS. For callus induction, explants were cultured on MS medium supplemented with NAA, 2,4-D and BA. For somatic embryogenesis, the calli were cultured on MS medium supplemented with full, 1/4 and 1/10 fold of growth regulators and MS medium without growth regulator. For maturation, embryogenic clumps were transferred on MS medium supplemented with BA, 2,4-D and 30 or 60 g l-1 sucrose. The small embryogenic clumps were transferred to medium with GA3.
Results: The main components of essential oils were Ocimene (29.84%), followed by α-pinene (27.01%), Isobornyl formate (7.7%) and gamma-Terpinene (4.85%). The highest percentage of callus induction observed in the root and hypocotyl explants. The maximum somatic embryogenesis was obtained in MS medium with 0.5 mg l-1 2, 4-D via hypocotyls derived explants. The highest frequency of maturation was obtained in MS medium containing 30 g l-1 sucrose without growth regulators.
Conclution: This plant had a noticeable amount of essential oil with valuable ingredients and tissue culture techniques could be applied for its mass propagation, conservation of germplasm and in vitro regeneration.
Objective: The aim of this study was to achieve an efficient method for propagation of this plant and evaluation of essential oil content and composition in habitat.
Metthods: Essential oils were extracted by hydrodistillation using Clevenger-type apparatus and its composition was analyzed by the GC and GC-MS. For callus induction, explants were cultured on MS medium supplemented with NAA, 2,4-D and BA. For somatic embryogenesis, the calli were cultured on MS medium supplemented with full, 1/4 and 1/10 fold of growth regulators and MS medium without growth regulator. For maturation, embryogenic clumps were transferred on MS medium supplemented with BA, 2,4-D and 30 or 60 g l-1 sucrose. The small embryogenic clumps were transferred to medium with GA3.
Results: The main components of essential oils were Ocimene (29.84%), followed by α-pinene (27.01%), Isobornyl formate (7.7%) and gamma-Terpinene (4.85%). The highest percentage of callus induction observed in the root and hypocotyl explants. The maximum somatic embryogenesis was obtained in MS medium with 0.5 mg l-1 2, 4-D via hypocotyls derived explants. The highest frequency of maturation was obtained in MS medium containing 30 g l-1 sucrose without growth regulators.
Conclution: This plant had a noticeable amount of essential oil with valuable ingredients and tissue culture techniques could be applied for its mass propagation, conservation of germplasm and in vitro regeneration.
Type of Study: Research |
Subject:
Biotechnology
Received: 2017/02/9 | Accepted: 2017/07/5 | Published: 2017/08/21
Received: 2017/02/9 | Accepted: 2017/07/5 | Published: 2017/08/21
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