year 15, Issue 58 (4-2016)
J. Med. Plants 2016, 15(58): 182-188 |
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1- Department of Parasitology, School of Medicine, Zabol University of Medical Science, Zabol, Iran
2- Department of Medical Parasitology and Mycology, School of Medicine, Sanandaj University of Medical Sciences, Sanandaj, Iran ,maroofi.y@gmail.com
3- Department of Parasitology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
4- Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran
2- Department of Medical Parasitology and Mycology, School of Medicine, Sanandaj University of Medical Sciences, Sanandaj, Iran ,
3- Department of Parasitology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
4- Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran
Abstract: (6650 Views)
Background: Cutaneous leishmaniasis is one of major public health problem in many countries. Pentavalent antimonite components as first line treatment of cutaneous leishmaniasis has many limitations and side effects.
Objective: The aim of this study was to investigatethe cytotoxic effect of methanolic extract of Terminalia chebula Retz fruit on the Leishmania major in vitro.
Methods: Terminalia chebula Retz fruit extract with 80, 125, 250 and 500 µg/ml concentrations with leishmania promastigotes were added to 96-well plate as 4-time repeated and incubated in 21 ˚C. Viability percent of promastigotes was estimated by direct counting and MTT assay; after 24, 48 and 72 hours. Terminalia chebula Retz extract with80 and 250 μg/ml were added to infected macrophages. The mean of amastigotes in each macrophage was obtained after 24 and 48 hours. Statistical analysis of data was done by SPSS software and one-way ANOVA test.
Results: viability percent of promastigotes in groups treated by maximum and minimum concentrations (500 and 80 μg/ml) of Terminalia chebula Retz after 24 hours was 19.01% and 98.65%, respectively.Viability of this groups after 48hours was 7.81% and 97.26% and after 72 hours it was 31.61% and 97.26%, respectively. IC50 for 24 hours was 114.10 µg/ml. Cytotoxicity of Terminalia chebula Retz extract (80 and 250 μg/ml) in amastigotes after 24 hours was 40.35% and 29.99%; and after 48 hours was 45.21% and 41.11%, respectively.
Conclusion: extract of Terminalia chebula Retz fruit has cytotoxic effect on the promastigotes and amastigotes of L. major in vitro.
Objective: The aim of this study was to investigatethe cytotoxic effect of methanolic extract of Terminalia chebula Retz fruit on the Leishmania major in vitro.
Methods: Terminalia chebula Retz fruit extract with 80, 125, 250 and 500 µg/ml concentrations with leishmania promastigotes were added to 96-well plate as 4-time repeated and incubated in 21 ˚C. Viability percent of promastigotes was estimated by direct counting and MTT assay; after 24, 48 and 72 hours. Terminalia chebula Retz extract with80 and 250 μg/ml were added to infected macrophages. The mean of amastigotes in each macrophage was obtained after 24 and 48 hours. Statistical analysis of data was done by SPSS software and one-way ANOVA test.
Results: viability percent of promastigotes in groups treated by maximum and minimum concentrations (500 and 80 μg/ml) of Terminalia chebula Retz after 24 hours was 19.01% and 98.65%, respectively.Viability of this groups after 48hours was 7.81% and 97.26% and after 72 hours it was 31.61% and 97.26%, respectively. IC50 for 24 hours was 114.10 µg/ml. Cytotoxicity of Terminalia chebula Retz extract (80 and 250 μg/ml) in amastigotes after 24 hours was 40.35% and 29.99%; and after 48 hours was 45.21% and 41.11%, respectively.
Conclusion: extract of Terminalia chebula Retz fruit has cytotoxic effect on the promastigotes and amastigotes of L. major in vitro.
Type of Study: Research |
Subject:
Pharmacognosy & Pharmaceutics
Received: 2015/08/30 | Accepted: 2015/11/11 | Published: 2016/05/9
Received: 2015/08/30 | Accepted: 2015/11/11 | Published: 2016/05/9
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