year 12, Issue 45 (2-2013)                   J. Med. Plants 2013, 12(45): 29-42 | Back to browse issues page

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Ghorbanpour M, Ghafarzadegan R, Khavazi K, Hatami M. Two Main Tropane Alkaloids Variations of Black Henbane (Hyoscyamus niger) Under PGPRs Inoculation and Water Deficit Stress Induction at Flowering Stage. J. Med. Plants 2013; 12 (45) :29-42
URL: http://jmp.ir/article-1-107-en.html
1- Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University , m_ghorbanpour@yahoo.com
2- Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
3- Department of Soil Biology, Soil and Water Research Institute
4- Department of Horticultural Sciences, Faculty of Agriculture, Guilan University
Abstract:   (5336 Views)
Background: Plants infection with microorganisms as well as physical factors such as osmotic stresses especially drought stress induced particular secondary metabolite production. Objective: Plant root and shoot hyoscyamine (HYO) and scopolamine (SCO) variations were investigated under three water deficit stress (WDS) levels as 30 (W1), 60 (W2) and 90 % (W3) water depletion of field capacity and Pseudomonas putida (PP) and P. fluoresence (PF) strains inoculation at flowering stage. Methods: Seedling radicles and culture media were inoculated with PP and PF (109 CFU/ml) strains. Monitoring of soil moisture levels was conducted using gravimetric method. Alkaloids extracted were identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analysis. Results: Results revealed that HYO was the prevalent alkaloid in root and shoot organs. The highest ratio of SCO to HYO (0.53) and the highest alkaloids both HYO and SCO content in root and shoot was observed in W3PF treatment. The largest total alkaloids (HYO+SCO) yield (25.7 mg.plant-1) was showed in PP treated plants under W1 conditions. Conclusion: Integrative use of effective Pseudomonades strains and WDS sounds to be an encouraging and eco-friendly strategy for increasing tropane alkaloids yield and content in Hyoscyamus niger root and shoot parts.
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Type of Study: Research | Subject: Agriculture & Ethnobotany
Received: 2012/09/30 | Accepted: 2013/03/9 | Published: 2013/04/19

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