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Expression of Genes 1-deoxy-D-xylulose 5-phosphate Reductoisomerase (DXR), Sabinen Synthase (Tctps1) and α- terpineol Synthase (Tctps5) and its Relation to Thymol and Carvacrol Biosynthesis in Thymus vulgaris under Water Deficit Stress
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| F Mirzaei 1, F Fatehi 2, A Ismaili 3, A Yaghotipoor 4, A Qaderi * 5 |
1- 1- Agronomy and Plant Breeding Dept, Faculty of Agriculture, Lorestan University, Lorestan, Iran
2- 2- Payame Noor University (PNU), Tehran, Iran
3- 3- Faculty of Agriculture, Lorestan University, Lorestan, Iran
4- 4- Department of Agronomy and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
5- 5- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran |
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Abstract: (3036 Views) |
Background: Thymol and Carvacrol are the two important secondary metabolites from Thymus vulgaris that productions of them are controlled by genetic and environmental factors.
Objective: Evaluation of Water stress effects on expression of three important genes of thymol and carvacrol biosynthetic pathway and also physiological and phytochemical properties of Thymus vulgaris.
Methods: The present study was conducted in a randomized complete block design with 4 treatments and 3 replications in Institute of Medicinal Plants, ACECR, Karaj.
For this purpose, the treatments were selected as control (FC), 70% of FC, 40% of FC, and 20% of FC. Gene expression was studied using real-time PCR method, and HPLC was applied to essence analysis. Also physiological characteristic including chlorophyll content, relative water content, electrolyte leakage percentage and carotenoids content were analyzed.
Results: The results revealed that water stress significantly influenced the gene expression
(P≤ 0.05). The highest gene expression of DXR and Tctps5 was observed in 70% of FC, while the highest one of TvTPS1 was recorded in 40% of FC. Moreover, the maximum thymol and carvacrol was found in 70% of FC. In other hand the highest chlorophyll content and corotenoids content were obtained in 100% and 70% of FC respectively. Also there were not significant differences between treatments for relative water content and electrolyte leakage percentage.
Conclusion: The 70% of FC through influencing of genes in the firs and last of MEP pathway increased thymol and carvacrol production.
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| Keywords: Thymus vulgaris, Gene Expression, water deficit, Photosynthesis Pigments, thymol, carvacrol |
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Full-Text [PDF 450 kb]
(596 Downloads)
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Type of Study: Research |
Subject:
Agricultural Biotechnology
Received: 2017/11/6 | Accepted: 2017/11/6 | Published: 2017/11/6
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