en فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div style="text-align: justify;"><span style="font-size:14px;"><b><span new="" roman="" style="font-family:" times=""><span style="color:#222222">Background:</span></span></b><span new="" roman="" style="font-family:" times=""><span style="color:#222222"> The use of herbs and natural compounds in cosmetics is one of the most important issues for the treatment of skin disorders. In most cultures and societies, plants are traditionally used to treat skin diseases. Green tea or <i>Camellia sinensis</i> (L.) Kuntze, which belongs to the Theaceae family is a medeicinal herb which can be very practical in cosmetic formulations. <b>Objective:</b> In this study, hydroalcoholic green tea extract (2.5 %) has been applied for the preparation of herbal eye cream and then pharmaceutical and phytochemical properties of the product were evaluated. <b>Methods: </b>High-Performance Thin Layer Chromatography (HPTLC) and also spectrophotometric procedures were used for quantification of Epigallocatechin (as the main component in the extract), total phenol and flavonoid contents, respectively. On the other side, pharmaceutical evaluation tests including color and odor test, emulsion stability test, pH measurement, rheology and the total microbial count were investigated. <b>Results:</b> Epigallocatechin gallate, total phenolic compounds, and total flavonoid amounts of the final product have been calculated to be 13.02 &plusmn;</span><span style="color:black"> 0.05</span><span style="color:#222222">, </span><span style="color:black">198.35 &plusmn; 2.79</span><span style="color:#222222">, and </span><span style="color:black">19.56 &plusmn; 0.76</span><span style="color:#222222"> mg / 30 g product respectively.</span></span> <span lang="EN-CA"><span new="" roman="" style="font-family:" times=""><span style="color:black">After 6 months no changes in color, stability and amount of </span></span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">ingredients </span></span><span lang="EN-CA"><span new="" roman="" style="font-family:" times=""><span style="color:black">were observed. Regarding the microbial limit test, </span></span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">no signs of pathogen presence were observed.</span></span> <b><span lang="EN-CA"><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion</span></span></span><span new="" roman="" style="font-family:" times=""><span style="color:#222222">: </span></span></b><span new="" roman="" style="font-family:" times=""><span style="color:black">Based on the results of current investigation, this herbal formulation, with evaluated parameters and controlled items, could be considered as a perfect candidate for clinical evaluation in the field of periorbital cosmetics and anti-aging herbal eye cream.</span></span></span></div> Green tea extract, Herbal eye cream, Epigallocatechin, HPTLC, Anti-aging cream, Camellia sinensis 57 68 http://jmp.ir/browse.php?a_code=A-10-2501-7&slc_lang=en&sid=1 Shima Hashemi hashemi.sh66@gmail.com 100319475328460049691 0009-0007-6229-631X No Department of Pharmacognosy, Faculty of Pharmacy, Ramsar Campus, Mazandaran University of Medical Sciences, Ramsar, Iran Soliman Mohammadi Samani smsamani@sums.ac.ir 100319475328460049692 100319475328460049692 No Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Ardalan Pasdaran ardalan.pasdar@gmail.com 100319475328460049693 100319475328460049693 No Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Mohammad Mehdi Zarshenas zarm@sums.ac.ir 100319475328460049694 100319475328460049694 Yes Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran