en فارماكولوژی و سم شناسی Pharmacology & Toxicology پژوهشی Research <b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Background:</span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Multiple sclerosis (MS) is a chronic autoimmune disorder of the central nervous system that leads to inflammatory demyelination and axonal damage. <b>Objective</b>: This study evaluated the therapeutic potential of <i>Portulaca oleracea</i> extract in a mice model of CPZ-induced multiple sclerosis. <b>Methods:</b> In the present experiment, CPZ was used to induce an MS-like conditions in mice. During the final two weeks of CPZ exposure, mice received <i>P. oleracea</i> extract at doses of 100, 200, or 300 mg/kg/day by intraperitoneal injection. After the study period, behavioral tests, histochemical examination, and immunohistochemical analysis were performed to assess HO-1, NQO-1, p-Nrf2, and NF-&kappa;B p65. <b>Results:</b> Compared with control group, mice fed CPZ showed significant weight loss, impaired motor function, and significant demyelination of the corpus callosum in LFB staining. Administration of <i>P. oleracea</i> during the last two weeks improved behavioral deficits and reduced CPZ-associated weight loss and myelin damage. In the corpus callosum, CPZ decreased the expression of HO-1, NQO-1, and</span></span> <div style="text-align: justify;"><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p-Nrf2 while increasing the expression of NF-&kappa;B p65. These changes were partially reversed by treatment with the extract. <b>Conclusion:</b> <i>P.</i> oleracea may be a potential candidate for further preclinical research on MS.</span></span></div> Multiple sclerosis, Cuprizone, P. oleracea, Demyelination, Nrf2, NF-kB 1 16 http://jmp.ir/browse.php?a_code=A-10-7062-2&slc_lang=en&sid=1 Tayebeh Noori tahebehnoori94@gmail.com 100319475328460050144 100319475328460050144 No Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Seyede Zahra Hosseini zhosseini94@gmail.com 100319475328460050145 100319475328460050145 No Student Research Committee, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran Sina Korani sina.korani@yahoo.com 100319475328460050146 100319475328460050146 No Student Research Committee, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran Seyede Darya Alavi alavi_darya@yahoo.com 100319475328460050147 100319475328460050147 No Student Research Committee, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran Niloofar Heidarizade niloofar.heidarizade@gmail.com 100319475328460050148 100319475328460050148 No Student Research Committee, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran Ahmad Mohammadi-Farani ahmadmohammady@yahoo.com 100319475328460050149 100319475328460050149 No Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran; Department of Physiology and Pharmacology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran Samira Shirooie shirooie@gmail.com 100319475328460050150 100319475328460050150 Yes Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran