year 17, Issue 66 (5-2018)                   J. Med. Plants 2018, 17(66): 176-189 | Back to browse issues page

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Shooshtari L, Omidi M, Qaderi A, Zare Karizi A, Mehrafarin A. Genomic and Phytochemical Assessment of Chavir (Ferulago angulate) under Different in vitro Conditions. J. Med. Plants. 2018; 17 (66) :176-189
1- Department of Plant Breeding, College of Agriculture, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran ,
2- Department of Agronomy and Plant Breeding College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Abstract:   (2794 Views)
Background: Ferulago angulata (Known in Iran as Chavir) is an endangered species with Anti fugal and anti-bacterial activity that can act as a natural preservator.
Objective: Evaluation the effect of in vitro culture conditions and plant growth regulators on somaclonal variation and the phytochemical content of in vitro regenerated plants.
Methods: Induced calli were transferred to Murashige and Skoog (MS) medium supplemented with 1-Naphthaleneacetic acid (NAA) and 6-Benzylaminopurine (BAP) for regeneration stage. The essential oils were extracted by Clevenger apparatus and the yield and composition of essential oils were assayed by GC/Mass. Inter-Simple Sequence Repeat (ISSR), Start codon Targeted (SCoT) and CAAT Box Derived Polymorphism (CBDP) markers were used to assess somaclonal variation.
Results: The highest callus formation was obtained via radicle explant in MS medium supplemented with 2 mg/L 2,4-D and 1mg/L BAP. Maximum percentage of regeneration was occurred via derived calli from hypocotyl in MS medium supplemented with 0.1 mg/L BAP and 0.5 NAA. The phytochemical assay revealed a considerable differences between natural habitats and
in vitro regenerated plants. The percentage of
α-Pinene in plants derived from natural habitats and in vitro conditions were 27 and 1.53 respectively. The yield of essential oils were 2.26 and 0.64 (ml/100g D.M.) in natural habitats and in vitro regenerated plants respectively. The result of PCR assay indicated genetic variation between tissue cultured samples.
Conclusion: The results indicated that in vitro culture conditions had a considerable effect on genome and metabolome of Ferulago angulate.
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Type of Study: Research | Subject: Biotechnology
Received: 2017/08/26 | Accepted: 2018/01/14 | Published: 2018/06/24

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