دوره 1، شماره 65 - ( 12-1396 )                   جلد 1 شماره 65 صفحات 61-72 | برگشت به فهرست نسخه ها

XML English Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Esmaeilzadeh Bahabadi S, Rezaei A. Nitric Oxide Increased the Rosmarinic Acid and Essential Oil Production in In vitro-cultured Melissa officinalis. J. Med. Plants. 2018; 1 (65) :61-72
URL: http://jmp.ir/article-1-2086-fa.html
Esmaeilzadeh Bahabadi S، Rezaei A. Nitric Oxide Increased the Rosmarinic Acid and Essential Oil Production in In vitro-cultured Melissa officinalis. فصلنامه گياهان دارویی. 1396; 1 (65) :61-72

URL: http://jmp.ir/article-1-2086-fa.html


1- Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran
2- Department of Biotechnology, Faculty of Agriculture, Shahed University, Tehran, Iran
چکیده:   (13425 مشاهده)
Background: Nitric oxide is a free radical that recently has emerged as a key signaling molecule in regulating important physiological processes in plants.
Objective: In this work, sodium nitroprusside (SNP) was utilized as the donor of nitric oxide to investigate the effects of exogenous nitric oxide on essential oil, rosmarinic acid and antioxidant activity of in vitro-cultured Melissa officinalis plants.
Methods: The Plantlets were treated with different concentrations of SNP (0, 5, 10 and 20 mM) at eight leaf stages.
Results: The results showed that SNP decreased trans-caryophyllene and other selected essential oils in M. officinalis plants, while increased most of major components of essential oils. The highest content for linalool, neral and thymol was achieved at 5, 5 and 20 mM of SNP respectively. The highest monoterpene (56.17 % v/w) and sesquiterpene content (76.01 % v/w) were achieved by 5 and 20 mM SNP, respectively. In addition, the class of essential oil compounds varied depending on the SNP concentration. Application of SNP on culture media increased rosmarinic acid production and phenolic levels, which in turn improved the antioxidant properties of the extracts.
Conclusion: It seems that nitric oxide elicited M. officinalis culture and increased the secondary metabolite production. This current finding open new opportunities for obtaining valuable natural antioxidants for commercial exploitation by using tissue culture systems.
متن کامل [PDF 231 kb]   (636 دریافت)    
نوع مطالعه: پژوهشی |
دریافت: ۱۳۹۶/۱۲/۱۹ | پذیرش: ۱۳۹۶/۱۲/۱۹ | انتشار: ۱۳۹۶/۱۲/۱۹

ارسال نظر درباره این مقاله : نام کاربری یا پست الکترونیک شما:
CAPTCHA

کلیه حقوق این وب سایت متعلق به فصلنامه علمی پژوهشی گیاهان دارویی می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2020 All Rights Reserved | Journal of Medicinal Plants

Designed & Developed by : Yektaweb