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:: Volume 4, Number 56 (12-2015) ::
JMP 2015, 4(56): 78-86 Back to browse issues page
Effect of Hydro Alcoholic Extract of Nutmeg on Immune Response of Rat
M Ghorbanpoor *1, A Rahnama2, H Najafzadeh Varzi3, S Esmailzadeh4, Pourmahdi Broujeni M5
1- (Ph.D.) 1- Professor of Pathobiology Department, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran , m.ghorbanpoor@scu.ac.ir
2- (D.V.M. Graduate) 2- Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3- (Ph.D.) 3- Professor of Basic Sciences Department, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4- (Ph.D.) 4- Associate Professor of Pathobiology Department, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
5- (Ph.D.) 5- Associate Professor of Food Hygiene Department, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (1455 Views)

Background: Myristica fragrans is an evergreen aromatic tree cultivated in many tropical countries. Nutmeg, the dried ripe seed of M. fragrans, is a popular spice used in sweet and savory cooking, and a variety of drinks.

Objective: Nutmeg has a variety of pharmaceutical effects and in this study its effects on immune responses were evaluated.

Methods: Six groups (each group 8) of Wistar rats were treated as follows: Even groups received Nutmeg extract at dose 300 mg per kg of body weight intraperitoneally (IP), daily up to 12 days. Odd groups received PBS instead of nutmeg extract. The rats of groups 1 and 2 were immunized IP with 1.35×109 Sheep RBC (SRBC) in days 1 and 6. The rats of groups 3 and 4, were immunized IP with 1.35×109 SRBC in days 1 and subcutaneously in right foot pad with 2.7×107 SRBC in a volume of 0.1 ml on the day 9.  Titer of anti-SRBC antibody in the groups 1 and 2 and lysozyme and alternative complement pathway activity of groups 5 and 6 were evaluated on day 13. The swelling of footpads in groups 3 and 4 were measured at 24, 48, and 72 hours after challenge with SRBC and these rats euthanized on day 13 and theirs foot were examined histopathologically for infiltration of inflammatory cells.

Results: The result showed that nutmeg extract significantly increase anti SRBC titer (P=0.005), inhibit inflammatory cells infiltration (P< 0.001) but has not any effect on serum lysozyme or complement activity (P=0.4).

Conclusion: In conclusion nutmeg extract shows a significant suppression on cell mediated immunity and stimulatory effect on humeral immune response to SRBC in Wistar rats.

Keywords: Immune Response, Nutmeg, Rat
Full-Text [PDF 661 kb]   (606 Downloads)    
Type of Study: Research | Subject: Pathobiology
Received: 2016/02/13 | Accepted: 2016/02/13 | Published: 2016/02/13
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Ghorbanpoor M, Rahnama A, Najafzadeh Varzi H, Esmailzadeh S, Pourmahdi Broujeni M. Effect of Hydro Alcoholic Extract of Nutmeg on Immune Response of Rat. JMP. 2015; 4 (56) :78-86
URL: http://jmp.ir/article-1-1227-en.html
Volume 4, Number 56 (12-2015) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
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